Purification and spectroscopic characterization of photosystem II reaction center complexes isolated with or without Triton X-100.

@article{Eijckelhoff1996PurificationAS,
  title={Purification and spectroscopic characterization of photosystem II reaction center complexes isolated with or without Triton X-100.},
  author={Camiel Eijckelhoff and Henny van Roon and Marie Louise Groot and Rienk van Grondelle and Jan P. Dekker},
  journal={Biochemistry},
  year={1996},
  volume={35 39},
  pages={12864-72}
}
The pigment composition of the isolated photosystem II reaction center complex in its most stable and pure form currently is a matter of considerable debate. In this contribution, we present a new method based on a combination of gel filtration chromatography and diode array detection to analyze the composition of photosystem II reaction center preparations. We show that the method is very sensitive for the detection of contaminants such as the core antenna protein CP47, pigment-free and… CONTINUE READING