Purification and spectroscopic characteristics in N-methylformamide of the Azotobacter vinelandii Fe-Mo cofactor.

@article{Frank1989PurificationAS,
  title={Purification and spectroscopic characteristics in N-methylformamide of the Azotobacter vinelandii Fe-Mo cofactor.},
  author={Patrick Frank and S F Gheller and William E. Newton and Keith O Hodgson},
  journal={Biochemical and biophysical research communications},
  year={1989},
  volume={163 2},
  pages={746-54}
}
The iron-molybdenum cofactor from Azotobacter vinelandii can be removed from significant amounts of extraneous iron and other contaminants using anaerobic gel filtration. Electronic absorption spectra of the so-purified FeMoco along with analysis of the so-called 'easily complexed' iron are suggestive that FeMoco occupies at least two different states in N… CONTINUE READING