Purification and some properties of myeloperoxidase and eosinophil peroxidase from human blood.

@article{Olsen1983PurificationAS,
  title={Purification and some properties of myeloperoxidase and eosinophil peroxidase from human blood.},
  author={R. L. Olsen and C. Little},
  journal={The Biochemical journal},
  year={1983},
  volume={209 3},
  pages={
          781-7
        }
}
Myeloperoxidase and eosinophil peroxidase have been isolated from outdated human blood. Peroxidase activity was extracted from washed leucocytes using 0.5 M-CaCl2 and the extract further purified by chromatography on concanavalin A--Sepharose, phenyl-Sepharose and finally by gel filtration. The final enzyme preparations were highly purified according to spectral and gel-electrophoretic criteria. Under reducing and denaturing conditions on polyacrylamide-gel electrophoresis myeloperoxidase gave… Expand
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