Purification and some properties of cytosine deaminase from Salmonella typhimurium.

  title={Purification and some properties of cytosine deaminase from Salmonella typhimurium.},
  author={Thomas P. West and Mark S. Shanley and Gerard A. O'donovan},
  journal={Biochimica et biophysica acta},
  volume={719 2},
Purification and Properties of Extracellular Cytosine Deaminase from Chromobacterium violaceum YK 391
Cytosine deaminase (cytosine aminohydrolase, EC stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. The
Purification and Characterization of Thermostable Cytosine Deaminase from Aspergillus fumigatus
Preliminary results suggest that Aspergillus fumigatus CDA could be a scaffold for further in vivo studies on cancer prodrug-mediated therapies, or gene therapy applications.
Purification and characterization of Aspergillus parasiticus cytosine deaminase for possible deployment in suicide gene therapy
Cytosine Deaminase from Aspergillus parasiticus was purified and characterized and can be compared with CDs from other sources that are used in suicide gene therapy.
Cloning, overexpression, and purification of cytosine deaminase from Saccharomyces cerevisiae.
A purification protocol has been developed which permits recovery of 60% of cytosine deaminase in active form from induced cell lysates after two purification steps, which will be useful for isolating large quantities of pure enzyme which are required for the preclinical evaluation of monoclonal antibody-cytosineDeaminase conjugates in combination with 5-fluorocytosines.
Chemical modification of intracellular cytosine deaminase fromChromobacterium violaceum YK 391
Results suggested that cysteine, tryptophan and methionine residues might be located in or near the active sites of the enzyme, while serine and lysine were indirectly involved in the enzymic activity.
Cloning, Characterization and Bioinformatics Analysis of Novel Cytosine Deaminase from Escherichia coli AGH09
The results revealed that the Escherichia coli AGH09 CDase, a strong CDase producing strain, was isolated from the urinary infection patients and showed no signal sequences and disulfide bond along with no allergenicity, suggested that the enzyme might be a novel CDase for deployment in cancer treatment.
Cytosine deaminase that hydrolyzes creatinine to N-methylhydantoin in various cytosine deaminase-forming microorganisms
The wide occurrence of cytosine deaminases in microorganisms was found to be related to the wide distribution of those microorganisms which hydrolyze creatinine to N-methylhydantoin without further degradation.


Kinetic Properties of Cytosine Deaminase from Pseudomonas aureofaciens
Some of the kinetic properties of purified cytosine deaminase from Pseudomonas aureofaciens were studied and this enzyme was inhibited strongly by 1 mm p-chloromercuribenzoate and seemed to be a thiol enzyme.
Kinetic Properties of Cytosine Deaminase from Serratia marcescens
The enzyme activity was affected by various nucleotides: strongly activated by IDP, ITP, GTP and ATP, and inhibited by deoxyguanosine and deoxyGMP.
Salmonella typhimurium Mutants Defective in Cytidine Monophosphate Kinase (cmk)
The mutant phenotype suggests that the physiological role of CMP kinase is that of rephosphorylating CMP arising from the breakdown of messenger ribonucleic acid, supported by the fact that a cmk(-) mutant is much more sensitive to any partial impairment of cytidine 5'-triphosphate synthetase than is thecmk(+) parent strain.
Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine
Quantitative nucleotide pool determinations have provided evidence that any individual ribo- or deoxyribonucleoside mono-, di-, or triphosphate of cytosine or uracil is not a repressing metabolite for the pyrimidine biosynthetic enzymes.
Pyrimidine nucleotide metabolism and pathways of thymidine triphosphate biosynthesis in Salmonella typhimurium.
  • J. Neuhard
  • Biology, Chemistry
    Journal of bacteriology
  • 1968
The nucleoside triphosphate pools of two cytidine auxotrophic mutants of Salmonella typhimurium LT-2 were studied under different conditions of pyrimidine starvation, and it was concluded that only cytidine nucleotides participate in the feedback regulation of de novo synthesis of UTP.
Binding of human interferons to immobolized Cibacron Blue F3GA: The nature of molecular interaction.
Blue Dextran (Cibacron Blue F3GA-dextran) was immobilized on cyanogen bromide activated agarose and used as a ligand for human fibroblast and leukocyte interferons in a solvent of phosphate-buffered