Purification and some properties of NAD-degrading purine nucleosidase from Aspergillus niger.

@article{Kuwhara1978PurificationAS,
  title={Purification and some properties of NAD-degrading purine nucleosidase from Aspergillus niger.},
  author={M. Kuwhara and T. Fujii},
  journal={Canadian journal of biochemistry},
  year={1978},
  volume={56 5},
  pages={
          345-8
        }
}
An enzyme which degrades NAD at the adenine-ribose linkage has been purified from the mycelial extract of Aspergillus niger. NADP, deamido-NAD, and purine nucleosides and nucleotides were also susceptible to the hydrolytic cleavage. Pyrimidine- and nicotinamide-ribose linkages were not attacked. The substrate specificity showed that the enzyme may be classified as a N-ribosyl-purine ribohydrolase (EC 3.2.2.1). The enzyme had a maximum activity in the pH range of 4.0-4.5 toward NAD. The Km… Expand
10 Citations
Hydrolysis of RNA monomers by extracts of Aspergillus niger NRRL3