Purification and refolding of recombinant Haemophilus influenzae type b porin produced in Bacillus subtilis.

@article{Dahan1996PurificationAR,
  title={Purification and refolding of recombinant Haemophilus influenzae type b porin produced in Bacillus subtilis.},
  author={Doz. Dr. J. Dahan and Ramakrishnan Srikumar and Raynald Laprade and James W. Coulton},
  journal={FEBS letters},
  year={1996},
  volume={392 3},
  pages={
          304-8
        }
}
The major diffusion channel in the outer membrane of Haemophilus influenzae type b (Hib) is porin (341 amino acids; Mr 37 782). The Hib porin gene was cloned and overexpressed in Bacillus subtilis. Recombinant Hib porin (Bac porin), having aggregated into inclusion bodies, was purified under denaturing conditions and subsequently refolded. To compare Bac porin that is intrinsically devoid of lipooligosaccharides versus native Hib porin, the properties of Bac porin were assessed by the following… CONTINUE READING