Purification and reconstitution of the sodium- and potassium-coupled glutamate transport glycoprotein from rat brain.

@article{Danbolt1990PurificationAR,
  title={Purification and reconstitution of the sodium- and potassium-coupled glutamate transport glycoprotein from rat brain.},
  author={Niels Christian Danbolt and Guy Pines and Baruch I. Kanner},
  journal={Biochemistry},
  year={1990},
  volume={29 28},
  pages={6734-40}
}
The sodium- and potassium-coupled L-glutamate transporter from rat brain has been purified to near homogeneity by reconstitution of transport as an assay, assuming that inactivated and active transporters cochromatograph. The purification steps involve lectin chromatography of the membrane proteins solubilized with 3-[(3-chloramidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), fractionation on hydroxylapatite, and ion-exchange chromatography. The specific activity is increased 30-fold. The… CONTINUE READING
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