Purification and radioimmunoassay of rat lactate dehydrogenase A and B subunits.

@article{Beebee1982PurificationAR,
  title={Purification and radioimmunoassay of rat lactate dehydrogenase A and B subunits.},
  author={T J Beebee and D S Carty},
  journal={The Biochemical journal},
  year={1982},
  volume={205 2},
  pages={313-20}
}
We have developed procedures for purifying lactate dehydrogenase isoenzymes from rat tissues that involve two affinity-chromatography steps and that facilitate the isolation of milligram quantities of highly purified proteins within 2--3 days. Antibodies raised against pure A and B subunits in rabbits and hens were used in radioimmunoassays and showed… CONTINUE READING