Purification and properties of yeast ATP (CTP):tRNA nucleotidyltransferase from wild type and overproducing cells.

@article{Chen1990PurificationAP,
  title={Purification and properties of yeast ATP (CTP):tRNA nucleotidyltransferase from wild type and overproducing cells.},
  author={Jia Yan Chen and Gabriele Kirchner and Markus Aebi and Nancy C. Martin},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 27},
  pages={16221-4}
}
ATP (CTP):tRNA nucleotidyltransferase (EC 2.7.7.25) has been purified from wild type cells of the yeast Saccharomyces cerevisiae, as well as from a strain that overproduces the activity. Purification from the wild type strain was accomplished with a multistep protocol including ammonium sulfate fractionation, anion exchange chromatography, gel filtration… CONTINUE READING