Purification and properties of uroporphyrinogen III synthase from human erythrocytes.

@article{Tsai1987PurificationAP,
  title={Purification and properties of uroporphyrinogen III synthase from human erythrocytes.},
  author={Shih Feng Tsai and David F. Bishop and Robert J Desnick},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 3},
  pages={1268-73}
}
Uroporphyrinogen III synthase (hydroxymethylbilane hydro-lyase (cyclizing); EC 4.2.1.75), the fourth enzyme in the heme biosynthetic pathway, was purified to homogeneity from human erythrocytes. For enzyme purification and characterization, a sensitive coupled enzyme assay was used which generated the substrate, hydroxymethylbilane; the oxidized product… CONTINUE READING