Purification and properties of three types of xylanases produced by an alkalophilic actinomycete

@article{Tsujibo1990PurificationAP,
  title={Purification and properties of three types of xylanases produced by an alkalophilic actinomycete},
  author={Hiroshi Tsujibo and T Sakamoto and Norikazu Nishino and Tōru Hasegawa and Yoshihiko Inamori},
  journal={Journal of Applied Microbiology},
  year={1990},
  volume={69},
  pages={398-405}
}
Three types of xylanases (l,4-β-D-xylan xylanohydrolase, EC 3.2.1.8) were isolated from the culture filtrate of an alkalophilic actinornycete, Nocardiopsis dassonvillei subsp. alba OPC-18. The enzymes (X-I, X-II and X-III) were purified by acetone precipitation, chromatographies of DEAE-cellulofine A-800, Sephadex G-75 and preparative isoelectric focusing. The purified enzymes showed single bands on sodium dodecyl sulphate polyacrylamide gel electrophoresis. The molecular weights of X-I, X-II… 
Purification and Characterization of Xylanases from Streptomyces sp. PC22
Two xylanases, namely xylanase I and xylanase II from Streptomyces sp. PC22 were purified from culture filtrate to 13.6- and 17.3- fold of purity, respectively by ammonium sulfate precipitation,
Purification, properties, and partial amino acid sequences of thermostable xylanases from Streptomyces thermoviolaceus OPC-520
TLDR
Two types of xylanases isolated from the culture filtrate of a thermophilic actinomycete, Streptomyces thermoviolaceus OPC-520, degraded xylan, producing xylose and xylobiose as the predominant products, indicating that they were endoxylanases.
Purification and Properties of a Xylan-Binding Endoxylanase from Alkaliphilic Bacillus sp. Strain K-1
TLDR
One of the extracellular xylanases that is stable in an alkaline state was purified to homogeneity by affinity adsorption-desorption on insoluble xylan, and it was demonstrated that the enzyme could hydrolyze the raw lignocellulosic substances effectively.
Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6
TLDR
Xylan completely protected the protein from inactivation by N-bromosuccinimide, and its activity was strongly inhibited by Zn2+, Cd2+, and Hg2+.
Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1
TLDR
Xylanase J showed high sequence homology with the xylanases from Bacillus pumilus and Clostridium acetobutylicum in the N-terminal region, indicating a possible application of the enzyme in biobleaching processes.
Production, purification, characterization and over-expression of xylanases from actinomycetes.
TLDR
This review provides the state-of-art information and developments on the xylanases from actinomycetes, presenting the production, purification, characterization and over-expression from various act inomycETes cultures.
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TLDR
Three types of enzyme from the culture filtrate have been purified by ultrafiltration with DIAFLO UM-10, chromatography on DEAE-Sephadex A-25, gel filtration on Bio Gel P-100, and isoelectric focusing with Servalyt 6~8 or 9~11.
Purification and characterization of xylanases from alkalophilic thermophilic Bacillus spp.
Xylanases from alkalophilic thermophilic Bacillus spp. W1 and W2 were purified and characterized. The xylanases from the two strains were fractionated into two active components (I and II) by
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TLDR
Immunological experiments indicated that xylanase A and xylan enzyme N were entirely different protein molecules, and both enzymes split xylan and yielded xylobiose and higher oligosaccharides but could hydrolyze neither xylanobiose nor xylotriose.
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Patterns of hydrolysis demonstrate that the three enzymes are endo-splitting enzymes able to break down xylan at random giving xylobio...
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
Five different xylanases and a β-D-xylosidase in the culture medium of Aspergillus niger have been purified to homogeneity from 13- to 52-fold by a procedure of gel and hydroxylapatite chromatography, and all seemed to be capable of liberating L-arabinose from either arabinoxylan or the arabinose–xylose oligosacc...
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