Purification and properties of thiosulfate dehydrogenase from Acidithiobacillus thiooxidans JCM7814.

@article{Nakamura2001PurificationAP,
  title={Purification and properties of thiosulfate dehydrogenase from Acidithiobacillus thiooxidans JCM7814.},
  author={Kazuo Nakamura and Masatoshi Nakamura and Hanae Yoshikawa and Yoshifumi Amano},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2001},
  volume={65 1},
  pages={102-8}
}
A key enzyme of the thiosulfate oxidation pathway in Acidithiobacillus thiooxidans JCM7814 was investigated. As a result of assaying the enzymatic activities of thiosulfate dehydrogenase, rhodanese, and thiosulfate reductase at 5.5 of intracellular pH, the activity of thiosulfate dehydrogenase was measured as the key enzyme. The thiosulfate dehydrogenase of A. thiooxidans JCM7814 was purified using three chromatographies. The purified sample was electrophoretically homogeneous. The molecular… CONTINUE READING