Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae.

@article{Dake1988PurificationAP,
  title={Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae.},
  author={E Dake and Timothy J. Hofmann and Steven L. McIntire and A. K. Hudson and Hans Zassenhaus},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 16},
  pages={7691-702}
}
The vast majority of nuclease activity in yeast mitochondria is due to a single polypeptide with an apparent molecular weight of 38,000. The enzyme is located in the mitochondrial inner membrane and requires non-ionic detergents for solubilization and activity. A combination of heparin-agarose and Cibacron blue-agarose chromatography was employed to purify the nuclease to approximately 90% homogeneity. The purified enzyme shows multiple activities: 1) RNase activity on single-stranded, but not… CONTINUE READING
20 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

Similar Papers

Loading similar papers…