Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu.

@article{Koch1986PurificationAP,
  title={Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu.},
  author={Christian Koch and Regine Kahmann},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 33},
  pages={15673-8}
}
G inversion in bacteriophage Mu requires the product of the DNA invertase gene gin and an Escherichia coli host factor termed FIS (factor for inversion stimulation). A recombination substrate must contain two recombination sites, arranged as inverted repeats, and a recombinational enhancer sequence termed sis. FIS has been purified to homogeneity. The purified protein has a relative molecular weight of 12,000 when analyzed under denaturing conditions. The intact protein behaves as a dimer of… CONTINUE READING

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