• Corpus ID: 6342008

Purification and properties of geranylgeranyl-diphosphate synthase from bovine brain.

  title={Purification and properties of geranylgeranyl-diphosphate synthase from bovine brain.},
  author={Hiroshi Sagami and Y Morita and Kyozo Ogura},
  journal={The Journal of biological chemistry},
  volume={269 32},
Geranylgeranyl-diphosphate synthase was purified to homogeneity from bovine brain in a one-step procedure employing an affinity column. For the construction of the affinity column, a farnesyl diphosphate analog, O-(6-amino-1-hexyl)-P-farnesylmethyl phosphonophosphate, was synthesized and linked to the spacer of the matrix of Affi-Gel 10 via the amino group. The native enzyme appeared to be a homooligomer (150-195 kDa) with a molecular mass of the monomer of 37.5 kDa. The pI for the enzyme was 6… 
Human geranylgeranyl diphosphate synthase: isolation of the cDNA, chromosomal mapping and tissue expression.
The nucleotide sequence of human geranylgeranyl diphosphate (GGPP) synthase cDNA isolated from a fetal heart library is established that the cDNA encodes the mammalian GGPP synthase protein.
Partial purification of a farnesyl diphosphate synthase from whole-body Manduca sexta.
  • S. Sen, A. Sperry
  • Biology, Chemistry
    Insect biochemistry and molecular biology
  • 2002
Human Geranylgeranyl Diphosphate Synthase
The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGP PSase, which differs immunochemically from bovines brain FPPSase.
Human geranylgeranyl diphosphate synthase is an octamer in solution.
Results indicate that the active form of GGPS in the solution is an octamer rather than hexamer or dimer.
Polyprenyl diphosphate synthases.
It is noteworthy that in spite of the similarity of the reactions catalyzed by these prenyltransferases, the modes of expression of catalytic function are surprisingly different, varying according to
Cloning, expression and characterization of an insect geranylgeranyl diphosphate synthase from Choristoneura fumiferana.