Purification and properties of rat small intestinal arginase.


Arginase [L-arginine amidinhydrolase EC] from rat small intestine was purified about 2,200-fold and its properties were compared with those of the rat liver and kidney enzymes. Intestinal arginase was extremely labile on storage either at -10 degrees or 4 degrees and lost activity during purification unless 25 mM L-valine was present. The purified… (More)