Purification and properties of phenylalanyl-tRNA synthetase from a higher plant (Phaseolus vulgaris).

Abstract

Phenylalanyl-tRNA synthetase from beans (Phaseolus vulgaris) was purified 2 800-fold to homogeneity with a 16% overall yield by salting-out chromatography, salting-out affinity chromatography, gel filtration and chromatography on DEAE-cellulose and hydroxylapatite. This combination minimizes potentially harmful effects of proteinases and products of the… (More)

Topics

Cite this paper

@article{Rauhut1984PurificationAP, title={Purification and properties of phenylalanyl-tRNA synthetase from a higher plant (Phaseolus vulgaris).}, author={Roman Rauhut and H J Gabius and Friedrich Cramer}, journal={Hoppe-Seyler's Zeitschrift fur physiologische Chemie}, year={1984}, volume={365 3}, pages={289-96} }