Purification and properties of phenylalanine ammonia-lyase from leaf mustard.


Phenylalanine ammonia-lyase (PAL, EC, the first enzyme in phenylpropanoid biosynthesis, catalyzes the elimination of ammonium ion from L-phenylalanine. In the present study, PAL was purified through ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-200 chromatography, and Q-Sepharose chromatography from the cytosolic… (More)


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