Purification and properties of leucine beta-naphthylamidase from rabbit small-intestinal mucosal cells.

@article{Takesue1975PurificationAP,
  title={Purification and properties of leucine beta-naphthylamidase from rabbit small-intestinal mucosal cells.},
  author={Yoshiko Takesue},
  journal={Journal of biochemistry},
  year={1975},
  volume={77 1?},
  pages={103-15}
}
Leucine beta-naphthylamidase associated with the microvilli membranes of rabbit small intestine was solubilized with papain [EC 3.4.22.2] and purified by Sephadex G-200 gel filtration, DEAE-cellulose column chromatography, passage through a column of Sepharose 4B coupled with anti-sucrase antibodies and preparative disc electrophoresis in polyacrylamide gel. The purified enzyme was homogeneous on ultracentrifugation and disc electrophoresis, but a double immunodiffusion test showed the presence… CONTINUE READING