Purification and properties of human glucose-6-phosphate dehydrogenase made in E. coli.

Abstract

The cDNA for the X-chromosome encoded human glucose-6-phosphate dehydrogenase (G6PD) has been expressed in E. coli and the enzyme purified to homogeneity, using a simple one-step fractionation on 2'5'-ADP-Sepharose. By selecting one of several different expression vectors and by optimizing culture conditions a yield of more than 10 mg of pure enzyme per… (More)

Topics

Cite this paper

@article{Bautista1992PurificationAP, title={Purification and properties of human glucose-6-phosphate dehydrogenase made in E. coli.}, author={J. M. Bautista and P. J. Mason and Lucio Luzzatto}, journal={Biochimica et biophysica acta}, year={1992}, volume={1119 1}, pages={74-80} }