Purification and properties of estrogen-responsive cytoplasmic thymidine kinase from human breast cancer.

@article{Bronzert1981PurificationAP,
  title={Purification and properties of estrogen-responsive cytoplasmic thymidine kinase from human breast cancer.},
  author={Diane Bronzert and Marie E. Monaco and L M Pinkus and stuart. aitken and Marc E. Lippman},
  journal={Cancer research},
  year={1981},
  volume={41 2},
  pages={604-10}
}
The effect of 17 beta-estradiol on cytoplasmic thymidine kinase activity was studied in MCF-7, a human breast cancer cell line in culture which responds to estrogens with an increase in the rate of growth. Levels of 17 beta-estradiol which maximally stimulate [3H]thymidine incorporation into DNA also maximally stimulate thymidine kinase activity. The Vmax for thymidine increased while the Km was not affected by estrogen stimulation when performed on nonpurified enzyme. Tamoxifen, an… CONTINUE READING
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