Purification and properties of cyclic-3',5'-GMP-dependent protein kinase from the nematode Ascaris suum.

Abstract

A cyclic-3',5'-GMP-dependent protein kinase was purified 7400-fold from the reproductive tract of female ascarids to a specific activity of 718 nmol min-1 mg-1 (histone as substrate). The yield of the preparation was 25%. The enzyme protein obtained was homogeneous as judged by isoelectrofocusing and polyacrylamide gel electrophoresis in the presence of… (More)

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@article{Thalhofer1989PurificationAP, title={Purification and properties of cyclic-3',5'-GMP-dependent protein kinase from the nematode Ascaris suum.}, author={H P Thalhofer and Heidi Hofer}, journal={Archives of biochemistry and biophysics}, year={1989}, volume={273 2}, pages={535-42} }