Purification and properties of aromatic amino acid aminotransferase from Klebsiella aerogenes.

@article{Paris1981PurificationAP,
  title={Purification and properties of aromatic amino acid aminotransferase from Klebsiella aerogenes.},
  author={C G Paris and Boris Magasanik},
  journal={Journal of bacteriology},
  year={1981},
  volume={145 1},
  pages={266-71}
}
We describe the complete purification of aromatic aminotransferase I, the enzyme responsible for the ability of Klebsiella aerogenes to use tryptophan and phenylalanine as sole sources of nitrogen, as well as the partial purification of aromatic aminotransferase IV. An examination of the properties of these enzymes revealed that aminotransferase I had much greater affinity for the aromatic amino acids than aminotransferase IV, explaining the essential role of aminotransferase I in the… CONTINUE READING