Purification and properties of an extracellular endoglucanase from Myceliophthora thermophila D-14 (ATCC 48104).

@article{Roy1990PurificationAP,
  title={Purification and properties of an extracellular endoglucanase from Myceliophthora thermophila D-14 (ATCC 48104).},
  author={Siddhartha Roy and Sudhansa K Dey and Sanghamitra Raha and Sajal Chakrabarty},
  journal={Journal of general microbiology},
  year={1990},
  volume={136 10},
  pages={1967-71}
}
An extracellular endoglucanase (1,4-beta-glucanohydrolase, EC 3.2.1.4) produced by Myceliophthora thermophila D-14 (ATCC 48104) has been purified to homogeneity by ammonium sulphate precipitation and two consecutive ion-exchange chromatographic steps on DEAE-Sephadex A-50 columns. The enzyme was purified 13.8-fold and was homogeneous by analytical PAGE and SDS-PAGE. It has a high apparent Mr, of about 100,000. The pH and temperature optima for its activity were 4.8 and 65 degrees C respectively… CONTINUE READING