Purification and properties of aldehyde dehydrogenase from Saccharomyces cerevisiae.

@article{Tamaki1977PurificationAP,
  title={Purification and properties of aldehyde dehydrogenase from Saccharomyces cerevisiae.},
  author={Nanaya Tamaki and Masami Nakamura and Kazumoto Kimura and Tokiko Hama},
  journal={Journal of biochemistry},
  year={1977},
  volume={82 1},
  pages={73-9}
}
A procedure for the purification of aldehyde dehydrogenase from bakers' yeast (Saccharomyces cerevisiae) is reported. Treatment with acid, heat and organic solvents was avoided and chromatographic and filtration techniques in the presence of phenylmethylsulfonylfluoride were mainly used. An affinity chromatography step using the reactive dye Cibacron blue F3G-A, which was covalently bound to Sepharose 4B, was found to be essential. The enzyme was bound to and then released from the dye. The… CONTINUE READING