Purification and properties of acid ribonucleases in human serum and leukocytes.

Abstract

Acid RNase was purified from normal human serum about 2400-fold by chromatography on phosphocellulose and Sephadex G-75 and rechromatography on Sephadex G-75. Assayed with yeast RNA as substrate, the enzyme showed the maximal activity at about pH 6.5 with sodium phosphate buffer. The reaction was activated by Na+, K+, and spermine, but it was not affected… (More)

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@article{Akagi1978PurificationAP, title={Purification and properties of acid ribonucleases in human serum and leukocytes.}, author={K. Akagi and M. Yamanaka and Kazunori Murai and Takanori Omae}, journal={Cancer research}, year={1978}, volume={38 7}, pages={2163-7} }