Purification and properties of a thermostable phytase from Bacillus sp. DS11

@inproceedings{Kim1998PurificationAP,
  title={Purification and properties of a thermostable phytase from Bacillus sp. DS11},
  author={Young-ok Kim and Hyung-Kwoun Kim and Kyung-Sook Bae and Ju-Hyun Yu and Tae-Kwang Oh},
  year={1998}
}
Bacillus species producing a thermostable phytase was isolated from the soil of a Korean cattle shed. An extracellular phytase from Bacillus sp. DS11 was purified to homogeneity by acetone precipitation and Phenyl-Sepharose, Resource S, and Superose 12 column chromatographies. Its molecular weight was estimated to be 44 kDa by SDS-polyacrylamide gel electrophoresis. Its optimum temperature for phytase activity was 70°C and about 50% of its original activity remained after incubation at 90°C for… CONTINUE READING

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