Purification and properties of a new cathepsin from rat liver.


1) A lysosomal protease, a new cathepsin that inactivates glucose-6-phosphate dehydrogenase [EC] and some other enzymes and differs from cathepsin B [EC] was purified about 2,200-fold from crude extracts of rat liver by cell-fractionation, freezing and thawing, acetone treatment, gel filtration, and DEAE Sephadex and CM-Sephadex column… (More)