Purification and properties of a beta-mannosidase from shrimp (Penaeus japonicus) hepatopancreas.

@article{Chuang1991PurificationAP,
  title={Purification and properties of a beta-mannosidase from shrimp (Penaeus japonicus) hepatopancreas.},
  author={N N Chuang and Bing Chen Yang},
  journal={Comparative biochemistry and physiology. B, Comparative biochemistry},
  year={1991},
  volume={98 4},
  pages={627-30}
}
A beta-mannosidase was purified ca 720-fold to homogeneity from Penaeus japonicus, with a final spec. act. of 252 U/mg of protein. 2. By using SDS-polyacrylamide gel electrophoresis, the monomers of shrimp beta-mannosidase were discovered to have mol. wts of 31,000 and those of human placental enzyme have similar mol. wts. 3.The shrimp beta-mannosidase has an isoelectric point (pI) of 5.6 +/- 0.1, and the human placental enzyme has an identical pI. Both enzymes were sialyated. 4.The shrimp beta… CONTINUE READING