Purification and properties of TrwB, a hexameric, ATP-binding integral membrane protein essential for R388 plasmid conjugation.

Abstract

TrwB is an integral membrane protein linking the relaxosome to the DNA transport apparatus in plasmid R388 conjugation. Native TrwB has been purified in monomeric and hexameric forms, in the presence of dodecylmaltoside from overexpressing bacterial cells. A truncated protein (TrwBDeltaN70) that lacked the transmembrane domain could be purified only in the… (More)

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@article{Hormaeche2002PurificationAP, title={Purification and properties of TrwB, a hexameric, ATP-binding integral membrane protein essential for R388 plasmid conjugation.}, author={Itsaso Hormaeche and Itziar Alkorta and Fernando Moro and Jos{\'e} Marıa Valpuesta and F{\'e}lix M Go{\~n}i and Fernando de la Cruz}, journal={The Journal of biological chemistry}, year={2002}, volume={277 48}, pages={46456-62} }