Purification and properties of Sandercyanin, a blue protein secreted in the mucus of blue forms of walleye, Sander vitreus

  title={Purification and properties of Sandercyanin, a blue protein secreted in the mucus of blue forms of walleye, Sander vitreus},
  author={Chi Li Yu and Daniel J Ferraro and S. Ramaswamy and Mark H. Schmitz and Wayne F. Schaefer and David Templeton Gibson},
  journal={Environmental Biology of Fishes},
A blue protein present in the mucus coating blue forms of walleye, Sander vitreus, was purified to homogeneity by a combination of ion exchange and hydrophobic interaction chromatography. The purified protein has a molecular mass of 87,850 and is a homotetramer with a subunit molecular mass of 21,836. Solutions of the protein are deep blue in color and show absorbance maxima at 383 and 633 nm, respectively. Acetone treatment of the protein releases a blue chromophore with the spectral… 
Structural heterogeneity in biliverdin modulates spectral properties of Sandercyanin fluorescent protein
The spectral properties and crystal structures of engineered monomeric Sandercyanin-BLA complexes are reported and a mechanism for modulating photo-states in BLA-bound lipocalins, known only in phytochromes till date is postulated.
Localization and seasonal variation of blue pigment (sandercyanin) in walleye (Sander vitreus)
Sandercyanin was found solely on dorsal surfaces of the fish and was localized to novel cells in the epidermis, similar in appearance to secretory sacciform cells, and concentrations were significantly higher in fish collected in summer versus other seasons.
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Disrupting Fluorescence by Mutagenesis in a Green Fluorescent Fatty Acid Binding Protein from a Marine Eel
Key insights are provided into the potential mechanism of fluorescence in this class of fluorescent fatty acid-binding proteins and the binding constants of bilirubin to Chlopsid FP and mutants, measured by fluorescence titration.
Genetic Differentiation between the Blue and the Yellow Phenotypes of Walleye (Sander vitreus): An Example of Parallel Evolution
The results suggest the colonization of each lake by a single group of walleye followed by the parallel origin of the blue phenotype, which could represent an excellent model for studying mechanisms underlying divergent selection and reproductive isolation.
Taxonomy, Distribution, and Evolution of the Percidae
An up-to-date account relating the results of the latest DNA sequence and morphological analyses to resolve the relationships of the family Percidae, including its component genera and species, and newly assembled distribution maps for the taxa.
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Contemporary walleye populations possess high levels of genetic diversity and divergence, despite habitat degradation and exploitation, and genetic and previously published tagging data indicate that natal homing and spawning site philopatry led to population structure.
Comparative Genetic Diversity, Population Structure, and Adaptations of Walleye and Yellow Perch Across North America
Results reveal broad-scale correspondence to isolation by geographic distance, however, their fine-scale population structures show less relationship, often with pronounced genetic differences among some nearby reproductive groups.
Sexual selection acting on a speciation trait in darters (Percidae: Etheostoma)
It is demonstrated that female E. barrenense discriminate between the orange and red body hues of conspecific males and exhibit an association preference for orange over red models, providing evidence for a link between sexual selection and behavioral isolation and by extension for a role of sexual selection in speciation.


The covalent protein structure of insecticyanin, a blue biliprotein from the hemolymph of the tobacco hornworm, Manduca sexta L.
The amino acid sequence has been determined for the insecticyanin from the hemolymph of the fifth instar larvae of the tobacco hornworm, Manduca sexta, containing two disulfide bridges and a single polypeptide chain of 189 amino acids.
Purification and Characterization of Biliverdin-binding Protein from Larval Hemolymph of the Swallowtail Butterfly, Papilio xuthus L.
The biliverdin-binding protein from the larval hemolymph of the swallowtail butterfly, Papilio xuthus L., was purified and characterized and showed sequence similarity to the bilin-bindingprotein from Pieris brassicae.
Purification and characterization of a biliverdin-associated protein from the hemolymph of Manduca sexta.
Proton magnetic resonance spectroscopic and absorption spectroscopy demonstrate that the bilin is the biliverdin IX gamma isomer.
The complete amino-acid sequence of the bilin-binding protein from Pieris brassicae and its similarity to a family of serum transport proteins like the retinol-binding proteins.
The amino-acid sequence from the bilin binding protein (BBP) of the butterfly Pieris brassicae has been determined and it is demonstrated that proteins from a wide variety of sources can be gathered into a "superfamily", namely human alpha 1-acid glycoprotein.
The lipocalin protein family: structure and function.
  • D. Flower
  • Biology, Chemistry
    The Biochemical journal
  • 1996
It is now clear that the lipocalins exhibit great functional diversity, with roles in retinol transport, invertebrate cryptic coloration, olfaction and pheromone transport, and prostaglandin synthesis.
Unravelling the structural chemistry of the colouration mechanism in lobster shell.
A review of progress concentrating on recent results but set in the context of more than 50 years of work is presented, including the first observation of single-crystal diffraction of the full 'alpha-crustacyanin' complex comprising 16 protein subunits and 16 bound AXT molecules at 5 A resolution.
Phenotypic variation of walleye, Sander vitreus, in Canadian Shield lakes: New insights on percid polymorphism
The existence of a blue form of walleye in lakes of the Canadian Shield provides new insights into percid polymorphism and gives important information about their occurrence outside the Great Lakes area.