Purification and properties of NAD(P)H: (quinone-acceptor) oxidoreductase of sugarbeet cells.

  title={Purification and properties of NAD(P)H: (quinone-acceptor) oxidoreductase of sugarbeet cells.},
  author={Paolo Trost and Patrizia Bonora and Sandra Scagliarini and Paolo Pupillo},
  journal={European journal of biochemistry},
  volume={234 2},
NAD(P)H:(quinone-acceptor) oxidoreductase [NAD(P)H-QR], a plant cytosolic protein, was purified from cultured sugarbeet cells by a combination of ammonium sulfate fractionation, FPLC Superdex 200 gel filtration, Q-Sepharose anion-exchange chromatography, and a final Blue Sepharose CL-6B affinity chromatography with an NADPH gradient. The subunit molecular mass is 24 kDa and the active protein (94 kDa) is a tetramer. The isoelectric point is 4.9. The enzyme was characterized by ping-pong… CONTINUE READING
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