Purification and properties of 3-ketothiolase fromAlcaligenes latus

Abstract

3-Ketothiolase was purified fromAlcaligenes latus by ion exchange chromatography and isoelectric focusing. The purified enzyme was a tetramer with identical subunits (Mr, 42,000). The kinetic data for cleavage and condensation reactions with 3-ketothiolase were obtained at various substrate concentrations, and compared with those from other PHB-accumulating… (More)
DOI: 10.1007/BF01080141

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@article{Maekawa2005PurificationAP, title={Purification and properties of 3-ketothiolase fromAlcaligenes latus}, author={Baku Maekawa and Naoyuki Koyama and Yoshiharu Doi}, journal={Biotechnology Letters}, year={2005}, volume={15}, pages={691-696} }