Purification and properties of 17 beta-hydroxy-C19-steroid dehydrogenases of adult male guinea pig kidney.

@article{Shen1978PurificationAP,
  title={Purification and properties of 17 beta-hydroxy-C19-steroid dehydrogenases of adult male guinea pig kidney.},
  author={Chien Celia Shen and Charles D. Kochakian},
  journal={Endocrinology},
  year={1978},
  volume={103 6},
  pages={2040-52}
}
The 17 beta-hydroxy-C19-steroid dehydrogenase activity of adult male guinea pig kidney was separated into one isolated and two contiguous enzymatically pure fractions by submitting the cytosol to (NH4)2SO4 (40-80% saturation) precipitation, Sephadex G-75 filtration, and DEAE-Sephadex A-50 and CM-Sephadex C-50 chromatography. Further DEAE-Sephadex A-50 and CM-Sephadex C-50 chromatography separated eight isozymes, which were divided into four groups in accordance with their behavior on… CONTINUE READING