Purification and peptide mapping of rat brain choline acetyltransferase.

Abstract

Acetyl-CoA:choline O-acetyltransferase (EC 2.3.1.6) has been purified approximately 17,000-fold from rat brain. The purification protocol included acid and NH4SO4 precipitation, followed by chromatography on CM-Sephadex, phenyl-Sepharose, Sephadex G-150, and blue dextran Sepharose. Two peaks of enzyme activity were detected after blue dextran Sepharose… (More)

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@article{Dietz1980PurificationAP, title={Purification and peptide mapping of rat brain choline acetyltransferase.}, author={Guenter Dietz and Paul M. Salvaterra}, journal={The Journal of biological chemistry}, year={1980}, volume={255 22}, pages={10612-7} }