Purification and partial characterization of lipoxygenase with dual catalytic activities from human term placenta.

@article{Joseph1993PurificationAP,
  title={Purification and partial characterization of lipoxygenase with dual catalytic activities from human term placenta.},
  author={P. Joseph and S. Srinivasan and A. Kulkarni},
  journal={The Biochemical journal},
  year={1993},
  volume={293 ( Pt 1)},
  pages={
          83-91
        }
}
Lipoxygenase possessing dual catalytic activities, i.e. dioxygenase and hydroperoxidase, was purified from the cytosols of term placentas from non-smoking women. Concanavalin A affinity chromatography followed by phenyl-Sepharose CL-4B chromatography resulted in the separation of one hydrophobic and one non-hydrophobic isoenzyme. The concanavalin A-purified enzyme was used in all subsequent experiments. The dioxygenase activity of the enzyme exhibited a Vmax. of 204.37 +/- 17.66 nmol/min per mg… Expand
Co-oxidative metabolism of 4-aminobiphenyl by lipoxygenase from soybean and human term placenta.
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Results suggest that the co-oxidation catalyzed by HTPLO may be the underlying biochemical mechanism responsible for the transplacental toxicity of 4-ABP. Expand
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The results suggest that hepatic lipoxygenase may play an important role in the metabolism of this hepatocarcinogen, using linoleic acid as a cosubstrate. Expand
The Inhibitory Effect of KCN, NaN3 and some Bivalent Ions on Lipoxygenase Activity of the Purified Human Placental
Lipoxygenase (LOX) catalyzes irreversible transfer of oxygen molecule to Arachidonic and Lin-oleic acid to produce 13 Hydroproxy Octadecadienoic acid. Recent studies showed that the involvement ofExpand
Co-oxidation of acrylonitrile by soybean lipoxygenase and partially purified human lung lipoxygenase.
  • P. Roy, A. Kulkarni
  • Chemistry, Medicine
  • Xenobiotica; the fate of foreign compounds in biological systems
  • 1999
TLDR
Human lung lipoxygenase (HLLO) was partially purified by concanavalin-A (Con-A) affinity chromatography that provided an easy and rapid one-step procedure for the removal of haemoglobin from cytosol and was a approximately 6-fold better catalyst than SLO in converting ACN to cyanide. Expand
Aflatoxin B1 epoxidation catalysed by partially purified human liver lipoxygenase.
  • S. Roy, A. Kulkarni
  • Chemistry, Medicine
  • Xenobiotica; the fate of foreign compounds in biological systems
  • 1997
TLDR
The results strongly suggest that lipoxygenase is capable of aflatoxin B1 metabolism and this may represent yet another pathway for the bioactivation of this hepatocarcinogen in the human liver. Expand
Hydroperoxidase activity of lipoxygenase: a kinetic study of isoproterenol oxidation.
TLDR
A plausible kinetic mechanism is proposed to explain the observed lag period and inhibition by high concentrations of H2O2. Expand
Lipoxygenase activity in rat dermis and epidermis: partial purification and characterization.
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Lipoxygenase (LOX) activity in epidermis and dermis was distributed among microsomal and cytosolic fractions and main reaction products indicated that this was 15-LOX. Expand
Oxidative metabolism of aflatoxin B1 by lipoxygenase purified from human term placenta and intrauterine conceptal tissues.
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The evidence presented here clearly demonstrates that placental LO is capable of epoxidation of AFB1, and similar results were observed with LO preparations of human intrauterine conceptal tissues at 8-10 weeks of gestation. Expand
Lipoxygenase-another pathway for glutathione conjugation of xenobiotics: A study with human term placental lipoxygenase and ethacrynic acid.
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The evidence gathered in this in vitro study suggests for the first time that lipoxygenase present in the human term placenta is capable of EA-SG formation, and it is proposed that free radicals are involved in the process ofEA- SG formation by HTPLO. Expand
Co-oxidation of all-trans retinol acetate by human term placental lipoxygenase and soybean lipoxygenase.
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Results suggest that t-RAc is metabolized via the lipoxygenase pathway, and may constitute an important part of the puzzle worthy of consideration in the understanding of retinoid teratogenicity in humans. Expand
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