Purification and partial characterization of two prolactin-like glycoprotein hormone complexes from the midpregnant mouse conceptus.

@article{Colosi1987PurificationAP,
  title={Purification and partial characterization of two prolactin-like glycoprotein hormone complexes from the midpregnant mouse conceptus.},
  author={Peter Colosi and L M Ogren and Gudmundur Thordarson and Frank J Talamantes},
  journal={Endocrinology},
  year={1987},
  volume={120 6},
  pages={2500-11}
}
Two PRL-like glycoprotein hormone complexes were purified from the medium of cultured mouse conceptuses from day 10 of pregnancy: mouse placental lactogen-I (mPL-I) (29-32K), and mPL-I (36.5-42K). Sodium dodecyl sulfate-gel electrophoresis revealed that mPL-I (36.5-42K) is a complex of five proteins with mol wt of 36.5K, 37.5K, 39K, 40.5K, and 42K. Deglycosylation with peptide: N-glycosidase F or trifluoromethanesulfonic acid produced a single 29K protein. mPL-I (36.5-42K) was also sensitive to… CONTINUE READING