Purification and partial characterization of the Mr 30,000 integral membrane protein associated with the erythrocyte Rh(D) antigen.

@article{Agre1987PurificationAP,
  title={Purification and partial characterization of the Mr 30,000 integral membrane protein associated with the erythrocyte Rh(D) antigen.},
  author={P. Agre and A. M. Saboori and A. Asimos and B. Smith},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 36},
  pages={
          17497-503
        }
}
  • P. Agre, A. M. Saboori, +1 author B. Smith
  • Published 1987
  • Medicine, Chemistry
  • The Journal of biological chemistry
  • Erythrocytes bearing the Rh(D) antigen have an Mr 30,000 integral membrane protein which can be surface-labeled with 125I and can be quantitatively immunoprecipitated from Triton X-100-solubilized spectrin-depleted membrane vesicles. The 125I-labeled Rh(D)-associated protein was purified to radiochemical homogeneity from membrane skeletons solubilized in sodium dodecyl sulfate and urea by hydroxylapatite chromatography, gel filtration, and preparative polyacrylamide gel electrophoresis. The Rh… CONTINUE READING
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