Purification and partial characterization of intact and truncated chitinase from Bacillus thuringiensis HZP7 expressed in Escherichia coli

Abstract

To ascertain the effect of chitin-binding domain (ChBD) and fibronectin type III domain (FN3) on the characterization of the intact chitinase from Bacillus thuringiensis. An intact chitinase gene (chi74) from B. thuringiensis HZP7 and its truncated genes (chi54, chi63 and chi66) were expressed in Escherichia coli BL21. The expression products were analyzed… (More)
DOI: 10.1007/s10529-015-1970-6

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@article{Sha2015PurificationAP, title={Purification and partial characterization of intact and truncated chitinase from Bacillus thuringiensis HZP7 expressed in Escherichia coli}, author={Li Sha and Ensi Shao and Xiong Guan and Zhipeng Huang}, journal={Biotechnology Letters}, year={2015}, volume={38}, pages={279-284} }