Purification and partial characterization of intestinal-like alkaline phosphatase in rabbit kidney.

@article{FujimoriArai1991PurificationAP,
  title={Purification and partial characterization of intestinal-like alkaline phosphatase in rabbit kidney.},
  author={Y Fujimori-Arai and Iwao Koyama and Kazuyuki Hirano and Yoshikatsu Sakagishi and Tsugikazu Komoda},
  journal={Archives of biochemistry and biophysics},
  year={1991},
  volume={284 2},
  pages={320-5}
}
Two types of alkaline phosphatase (AP) isozymes in rabbit kidney, a major intestinal-like type and a minor tissue-unspecific type, have been identified. The former enzyme was purified from rabbit kidney by immunoaffinity chromatography using monoclonal anti-human intestinal AP antibody. The purified enzyme yielded a single protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the apparent molecular size of its monomer subunit was found to be 72,000. Three amino acid… CONTINUE READING