Purification and molecular characterization of cGMP-dependent protein kinase from Apicomplexan parasites. A novel chemotherapeutic target.

Abstract

The trisubstituted pyrrole 4-[2-(4-fluorophenyl)-5-(1-methylpiperidine-4-yl)-1H-pyrrol-3-yl]pyridine (Compound 1) inhibits the growth of Eimeria spp. both in vitro and in vivo. The molecular target of Compound 1 was identified as cGMP-dependent protein kinase (PKG) using a tritiated analogue to purify a approximately 120-kDa protein from lysates of Eimeria… (More)

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@article{Gurnett2002PurificationAM, title={Purification and molecular characterization of cGMP-dependent protein kinase from Apicomplexan parasites. A novel chemotherapeutic target.}, author={Anne M Gurnett and Paul A. Liberator and Paula M Dulski and Scott P. Salowe and Robert G. K. Donald and Jennifer Weimer Anderson and Judyann Wiltsie and Carmen Diaz and Georgiana Harris and Ben Chang and Sandra J Darkin-Rattray and Bakela Nare and Tami M Crumley and Penny Sue Blum and Andrew S Misura and Tamas Tamas and Mohinder K Sardana and Jeffrey Yuan and Tesfaye Biftu and Dennis M Schmatz}, journal={The Journal of biological chemistry}, year={2002}, volume={277 18}, pages={15913-22} }