Purification and in vitro reconstitution of the essential protein components of an aromatic polyketide synthase.

@article{Carreras1998PurificationAI,
  title={Purification and in vitro reconstitution of the essential protein components of an aromatic polyketide synthase.},
  author={Christopher W Carreras and Chaitan Khosla},
  journal={Biochemistry},
  year={1998},
  volume={37 8},
  pages={2084-8}
}
A minimal set of proteins which catalyze the synthesis of aromatic polketides from malonyl CoA has been purified and partially characterized. Plasmid-encoded actinorhodin (act) ketosynthase/chain-length factor (KS/CLF) complex was purified from Streptomyces coelicolor CH999/pSEK38, and assayed with purified aromatic PKS holo-ACPs which were overproduced and purified from Escherichia coli and phosphopantetheinylated in vitro using purified E. coli holo-ACP synthase. When highly purified… CONTINUE READING
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