Purification and in vitro labeling of interferon from a human fibroblastoid cell line.

@article{Berthold1978PurificationAI,
  title={Purification and in vitro labeling of interferon from a human fibroblastoid cell line.},
  author={Wolfgang Berthold and Cuiwen Tan and Yves S H Tan},
  journal={The Journal of biological chemistry},
  year={1978},
  volume={253 14},
  pages={5206-12}
}
Interferon was produced from a clonal human fibroblastoid line. This cell line was derived from an established fibroblastoid culture treated with ethylmethane sulfonate and is capable of producing higher amounts human interferon than primary human fibroblast cultures. The interferon produced from this cell line was purified by concanavalin A and phenyl-Sepharose column chromatography followed by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified interferon was… CONTINUE READING