Purification and identification of positive regulators binding to a novel element in the c-Jun promoter.

@article{Jiang2008PurificationAI,
  title={Purification and identification of positive regulators binding to a novel element in the c-Jun promoter.},
  author={Daifeng Jiang and Yanwen Zhou and Robert A Moxley and Harry W. Jarrett},
  journal={Biochemistry},
  year={2008},
  volume={47 35},
  pages={9318-34}
}
A putative response element, GAGCCTC, was observed years ago in footprinting analysis of the c-jun promoter, and here we investigate its function in regulating c-jun expression and identify a protein complex that binds there. Electrophoretic mobility shift assays demonstrate a sequence-specific binding complex with this element in HEK293 cells. Additionally, unlabeled consensus AP-1 element DNA, but not a similar NF-jun element DNA, competes with complex formation. Mutations of this element… CONTINUE READING

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