Purification and identification of a cytokinin from moss callus cells

@article{Beutelmann1977PurificationAI,
  title={Purification and identification of a cytokinin from moss callus cells},
  author={Peter Beutelmann and L S Bauer},
  journal={Planta},
  year={1977},
  volume={133},
  pages={215-217}
}
A cytokinin was isolated from the culture medium of callus cells of the moss hybridFunaria hygrometrica (L.) Sibth xPhyscomitrium piriforme Brid. The purification procedure included ethyl-acetate extraction, silver-salt precipitation, crystallization as picrate, and ion exchange chromatography. The structure of the cytokinin was confirmed as N6−(Δ2-isopentenyl)adenine by means of gas chromatography and mass spectrometry. The concentration of the compound in the culture medium was determined at… CONTINUE READING

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