Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli.

@article{Haese1995PurificationAF,
  title={Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli.},
  author={Clivia Haese and A C Le Dain and Boris Martinac},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 31},
  pages={18329-34}
}
The large mechanosensitive ion channel (MscL) of Escherichia coli was expressed on a plasmid encoding MscL as a fusion protein with glutathione S-transferase in an Escherichia coli strain containing a disruption in the chromosomal mscL gene. After purification of the fusion protein using glutathione-coated beads, thrombin cleavage allowed recovery of the MscL protein. The purified protein was reconstituted into artificial liposomes and found to be fully functional when examined with the patch… CONTINUE READING
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