Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography.

@article{Dobrev2005PurificationAD,
  title={Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography.},
  author={Petre I Dobrev and Libor Havl{\'i}cek and Miroslav V{\'a}gner and Jiř{\'i} Malbeck and Miroslav Kam{\'i}nek},
  journal={Journal of chromatography. A},
  year={2005},
  volume={1075 1-2},
  pages={
          159-66
        }
}
A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors. 

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