Purification and crystallization of the entire recombinant subunit E of the energy producer A(1)A(o) ATP synthase.

Abstract

A(1)A(o) ATP synthases are the major energy producers in archaea. Subunit E of the stator domain of the ATP synthase from Pyrococcus horikoshii OT3 was cloned, expressed and purified to homogeneity. The monodispersed protein was crystallized by vapour diffusion. A complete diffraction data set was collected to 3.3 A resolution with 99.4% completeness using… (More)
DOI: 10.1107/S1744309110001016

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