Purification and concentration of mycobacteriophage D29 using monolithic chromatographic columns.

Abstract

Bacteriophages are used widely in many fields, and phages with high purity and infectivity are required. Convective interaction media (CIM) methacrylate monoliths were used for the purification of mycobacteriophage D29. The lytic phages D29 from bacterial lysate were purified primarily by polyethylene glycol 8000 or ammonium sulphate, and then the resulting phages were passed through the CIM monolithic columns for further purification. After the whole purification process, more than 99% of the total proteins were removed irrespective which primary purification method was used. The total recovery rates of viable phages were around 10-30%. Comparable results were obtained when the purification method was scaled-up from a 0.34 mL CIM DEAE (diethylamine) monolithic disk to an 8 mL CIM DEAE monolithic column.

DOI: 10.1016/j.jviromet.2012.07.016

Cite this paper

@article{Liu2012PurificationAC, title={Purification and concentration of mycobacteriophage D29 using monolithic chromatographic columns.}, author={Keyang Liu and Zhanbo Wen and Na Li and Wenhui Yang and Lingfei Hu and Jie Wang and Zhe Yin and Xiaokai Dong and Jinsong Li}, journal={Journal of virological methods}, year={2012}, volume={186 1-2}, pages={7-13} }