Purification and cloning of carp nephrosin, a secreted zinc endopeptidase of the astacin family.

@article{Hung1997PurificationAC,
  title={Purification and cloning of carp nephrosin, a secreted zinc endopeptidase of the astacin family.},
  author={Chia-Cheng Hung and Hao Ran Huang and Congyu Huang and Freesia L. Huang and Geen Dong Chang},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 21},
  pages={
          13772-8
        }
}
We have purified a secreted proteinase of 23 kDa from carp head kidney by sequential column chromatography on a Reactive Blue 72-agarose dye affinity column and an FPLC Mono-P column. The secretion of this proteinase from carp head kidney can be stimulated by high concentrations of potassium. Since the carp proteinase is present mainly in the head kidney, kidney, and spleen (all of which are lymphohematopoietic organs), it is named nephrosin. The carp nephrosin is most sensitive to metal… CONTINUE READING
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